4.1 Blood collection
Blood can be collected from several sites in the mouse including tail vein, saphenous vein, retro-orbital sinus, brachial vessels, vena cava or cardiac puncture. Always ensure complete hemostasis before returning the mouse to its home cage.
4.2 Urine collection
Spontaneous urination: The mouse is allowed to roam around on cling wrap layered around a glass slap, put into an empty clean cage. The mouse is removed as soon as it urinated and the voided urine is then aspirated with a pipette tip. Avoid contaminating urine with faeces.
Terminal urine collection after killing: Direct puncture of bladder after killing and open of the body
4.3 Ascites collection: Swab the enterocoelia with 70% ethanol. Insert the needle into the skin, then aspirate to get the ascites.
4.4 Pleural fluid collection: Swab the thoracic cavity with 70% ethanol. Insert the needle into the skin, then aspirate to get the Pleural fluid.
4.5 Bone marrow collection: Extracted the femur of the mice, cleaned the bone and then flushed the marrow out with PBS in an aspirating needle.
4.6. Semen collection: electroejaculation
4.7 Organ collection: Observe the body and organs of mice, detect the body weight, observe the tumor-forming ability, et al.